| | 1. In Foal Inc., Hemet, CA; Dr. Rick Beck. Oocyte shipments from a California facility to out of state laboratories for in-vitro ICSI (Intracytoplasmic Sperm Injection) procedure, maintained a constant temperature setting of 38.5° C.
2. Texas A&M University, College Station, TX; Dr. Katrin Hinrichs Shipment of various reproductive samples at differing temperature protocols. These included: Oocyte transportation, Embryo transportation & Ovary transportation at 38.5° C, 38.2° C and 33° C to 18° C ramp-down respectively.
3. Hartman Equine Reproduction Center, Whitesboro, TX; Dr. David Hartman Receiving and transferring oocytes and embryos, Transferring ICSI'ed embryos and previously frozen and thawed embryos.
4 Equine Reproduction Innovations, Wellington CO; Dr. Fernando Campos Return shipment of ICSI’ed oocytes and cleaved embryos at a incubation temperature to support continued growth.
5 Miner Institute, Chazy, NY, Ovary shipments with a controlled ramp-down temperature protocol to Texas A&M University.
6 Royal Vista Southwest, Purcell, OK; Dr. James Bailey and Dr. Ryan Coy Embryo temperature controlled protocol and holding test, included 40 mare embryos held for 24 hours and 48 hours at a specific ramp-down protocol, using Partner Animal health flush and holding medias. 85% of all transfers had a heart beat at 24 hrs of holding.
7 Henderson Veterinary Associates, Ltd. York, PA; Dr. Boyd Henderson Long term holding of chilled semen at 15° C.
8. Crimson Farm, Buellton, CA Long range successful semen holding in excess of 5 days and establishing pregnancies
9. Santolina Farm Inc. Cave Creek, AZ; Jack Farmer, Equine fertility Specialist Long term holding of chilled semen in excess of 5 days and establishing successful pregnancies Semen temperature ramp-down protocol in preparation for freezing. Flushed embryo cooling curve ramp-down protocol in preparation for shipment.
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